Microdetermination of Protein with Radiocopper and Gel Filtration.

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Gel filtration of sialoglycoproteins.

The role of sialic acid in the gel-filtration behaviour of sialoglycoproteins was investigated by using the separated isoenzymes of purified human liver alpha-L-fucosidase and several other well-known sialic acid-containing glycoproteins (fetuin, alpha1-acid glycoprotein, thyroglobulin and bovine submaxillary mucin). For each glycoprotein studied, gel filtration of its desialylated derivative g...

متن کامل

Protein binding of corticoids studied by gel filtration.

The ability of porous bodies to distinguish between molecules of different sizes has been repeatedly observed. If these porous bodies are devoid of charged groups, solutes placed on top of a column filled with such a material will be partitioned chiefly by molecular sieve effect (1). Molecules with a size above a limiting value will leave the column immediately after the displacement volume. Sm...

متن کامل

Gel-Filtration Chromatography.

Gel-filtration chromatography is a versatile method that permits the effective separation of biological molecules in high yield. This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical matrix types. Applications of the technique are described, with references to the scientific literature.

متن کامل

A gel filtration method for studies on protein iron-binding.

This communication reports the use of a gel filtration technique and radioactive iron for rapid determination of protein-iron binding in complex mixtures. Many methods have been used to investigate the characteristics of iron binding by mammalian serum. Calorimetric techniques have been used to measure the iron-binding capacities of whole seral. Electrophoretic2y3 and precipitation41s methods, ...

متن کامل

Microdetermination of protein nitrogen with a dumbbell diffusion apparatus.

The use of a two-chamber, sealed microdiffusion apparatus has been investigated for the determination of protein nitrogen on samples containing 0.5 mg. protein. The apparatus is constructed from a ground-glass ball-and-socket joint, and all operations including digestion are performed in the designed apparatus. The method has been applied to the determination of nitrogen in tobacco mosaic virus...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Acta Chemica Scandinavica

سال: 1962

ISSN: 0904-213X

DOI: 10.3891/acta.chem.scand.16-0782